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胰蛋白酶处理的鸡胚成纤维细胞中细胞表面糖蛋白的再生与细胞对基质的黏附之间的关系。

Relationship between regeneration of cell surface glycoproteins in trypsin-treated chick embryo fibroblasts and cell adhesion to the substratum.

作者信息

Vernay M, Cornic M, Aubery M, Bourrillon R

出版信息

Experientia. 1978 Jun 15;34(6):736-7. doi: 10.1007/BF01947292.

DOI:10.1007/BF01947292
PMID:658285
Abstract

The ability of cells to adhere to a substratum was altered by treatment with trypsin but was restored after a 1.5-h culture. A concomitant incorporation of [3H] leucine and [14C] glucosamine in the trypsin-sensitive cell surface glycoproteins was observed and almost reached a plateau within 1.50 h following the treatment with trypsin.

摘要

用胰蛋白酶处理后,细胞附着于基质的能力发生改变,但在培养1.5小时后恢复。观察到胰蛋白酶敏感的细胞表面糖蛋白同时掺入了[3H]亮氨酸和[14C]葡糖胺,在用胰蛋白酶处理后的1.50小时内几乎达到平台期。

相似文献

1
Relationship between regeneration of cell surface glycoproteins in trypsin-treated chick embryo fibroblasts and cell adhesion to the substratum.胰蛋白酶处理的鸡胚成纤维细胞中细胞表面糖蛋白的再生与细胞对基质的黏附之间的关系。
Experientia. 1978 Jun 15;34(6):736-7. doi: 10.1007/BF01947292.
2
Correlation between the regeneration of cell surface glycoproteins and the cell re-adhesion to the substratum in trypsin-treated chick fibroblasts at various stages of embryo development.
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[Differential effect of tunicamycin on glycosylation of cell surface glycoproteins from chick fibroblasts during embryo development (author's transl)].
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Correlation between changes in cell adhesion and the ratio of N- to O-linked glycopeptides during chick embryo development.鸡胚发育过程中细胞黏附变化与N-连接型和O-连接型糖肽比例之间的相关性。
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Incorporation of l-[3H]fucose and d-[3H]glucosamine into cell-surface-associated glycoconjugates in epidermis of cultured pig skin slices.将L-[3H]岩藻糖和D-[3H]葡糖胺掺入培养的猪皮肤切片表皮中与细胞表面相关的糖缀合物中。
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Glycoproteins and glycosaminoglycans synthesized by human keratinocytes in culture. Their role in cell-substratum adhesion.人角质形成细胞在培养中合成的糖蛋白和糖胺聚糖。它们在细胞与基质黏附中的作用。
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Growth of fibroblasts on linear and planar anchorages of limiting dimensions.成纤维细胞在有限尺寸的线性和平坦固定物上的生长。
Exp Cell Res. 1973 Sep;81(1):104-10. doi: 10.1016/0014-4827(73)90116-x.
3
The interactions of lectins with animal cell surfaces.凝集素与动物细胞表面的相互作用。
Int Rev Cytol. 1974;39:89-190. doi: 10.1016/s0074-7696(08)60939-0.
4
Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts.基质的细胞黏附对鸡胚成纤维细胞生长的影响。
Exp Cell Res. 1974 Apr;85(2):319-33. doi: 10.1016/0014-4827(74)90133-5.
5
Growth response to lectins in chick embryo cells at different stages of development.鸡胚细胞在不同发育阶段对凝集素的生长反应。
Cell Differ. 1976 Apr;5(1):27-35. doi: 10.1016/0045-6039(76)90012-9.
6
Stimulation of the biosynthesis of membrane glycoproteins from Zajdela ascites hepatoma cells by Robinia lectin.
Biochim Biophys Acta. 1976 Sep 7;443(3):375-86. doi: 10.1016/0005-2736(76)90457-0.
7
Cell surface and metabolic labelling of the proteins of normal and transformed chicken cells.正常和转化鸡细胞蛋白质的细胞表面标记与代谢标记
Biochim Biophys Acta. 1976 Jun 17;436(2):335-52. doi: 10.1016/0005-2736(76)90198-x.