Aubery M, Bourrillon R
Cell Differ. 1976 Apr;5(1):27-35. doi: 10.1016/0045-6039(76)90012-9.
We report here the effect of Robinia lectin and Concanavalin A on fibroblasts and liver cells from chick embryos between the 8th and the 20th day of development. This was observed in vitro after different times of cultivation. There is evidence that these lectins decrease cell number in cultures from young embryo cells but that they stimulate the proliferation of older embryo cells. The optimum concentration of either lectin was 3 mug/ml. No effect was observed on 12- and 14-day cells at the different concentrations of lectin used. The agglutination of fibroblasts by these lectins regularly decreased from the 8th to the 16th day of development. Liver cells however were agglutinated at no stage. These results could perhaps be explained in terms of cell surface changes either during the course of ontogeny or as a result of lectin treatment.
我们在此报告刺槐凝集素和伴刀豆球蛋白A对发育第8天至第20天鸡胚成纤维细胞和肝细胞的影响。这是在体外培养不同时间后观察到的。有证据表明，这些凝集素会减少幼胚细胞培养物中的细胞数量，但会刺激老龄胚细胞的增殖。两种凝集素的最佳浓度均为3微克/毫升。在所使用的不同凝集素浓度下，未观察到对12天和14天细胞有影响。这些凝集素对成纤维细胞的凝集作用在发育第8天至第16天期间有规律地降低。然而，肝细胞在任何阶段都未发生凝集。这些结果或许可以从个体发育过程中细胞表面的变化或凝集素处理的结果方面来解释。
