Stiehm E R, Sztein M B, Steeg P S, Mann D, Newland C, Blaese M, Oppenheim J J
Clin Immunol Immunopathol. 1984 Mar;30(3):430-6. doi: 10.1016/0090-1229(84)90028-x.
Expression of DR antigen on cord blood (neonatal) human monocytes using complement-mediated cytotoxicity with anti-DR alloantisera and fluorescent-activated cell sorting (FACS) utilizing a battery of anti-DR mouse monoclonal antibodies was assessed. Forty preparations of neonatal cord blood monocytes were purified by adherence and elution from plastic petri dishes: lymphocyte contamination was less than 5% as indicated by esterase and peroxidase stains and cell sizing. By cytotoxicity tests 22 +/- 5.5% (SD) of neonatal monocytes expressed DR compared to its expression on 78.6 +/- 3.1% of adult monocytes. By FACS analysis, the frequency of DR expression on neonatal monocytes was 19-33% compared to 71-82% for adult monocytes. Incubation of neonatal monocytes with concanavalin A (Con A) or phytohemagglutinin (PHA)-stimulated human peripheral blood mononuclear cell culture supernatants (lymphokine) or recombinant interferon-alpha (IFN-alpha) increased the expression of DR antigens in a dose- and time-dependent manner. A Con A-supplemented culture supernatant of unstimulated peripheral blood mononuclear cells had no effect on DR expression. Neonatal monocytes that were pretreated with anti-DR and complement in order to remove DR-positive cells were induced to express DR antigen after 2 days in culture with lymphokine. Thus DR-negative neonatal monocytes can be induced to express DR antigen. These results suggest a correctable maturational deficiency of neonatal monocytes. The inducibility of DR antigen expression by lymphokines and recombinant IFN-alpha suggests that they play an important role in the regulation of immune responses.
利用抗DR同种异体抗血清通过补体介导的细胞毒性以及使用一系列抗DR小鼠单克隆抗体的荧光激活细胞分选（FACS），评估了脐血（新生儿）人单核细胞上DR抗原的表达。通过从塑料培养皿中贴壁和洗脱纯化了40份新生儿脐血单核细胞制剂：酯酶和过氧化物酶染色以及细胞大小分析表明淋巴细胞污染小于5%。通过细胞毒性试验，与78.6±3.1%的成人单核细胞相比，22±5.5%（标准差）的新生儿单核细胞表达DR。通过FACS分析，新生儿单核细胞上DR表达的频率为19 - 33%，而成人单核细胞为71 - 82%。用伴刀豆球蛋白A（Con A）或植物血凝素（PHA）刺激的人外周血单核细胞培养上清液（淋巴因子）或重组干扰素-α（IFN-α）孵育新生儿单核细胞，以剂量和时间依赖的方式增加了DR抗原的表达。未刺激的外周血单核细胞的Con A补充培养上清液对DR表达没有影响。用抗DR和补体预处理以去除DR阳性细胞的新生儿单核细胞，在与淋巴因子培养2天后被诱导表达DR抗原。因此，DR阴性的新生儿单核细胞可以被诱导表达DR抗原。这些结果提示新生儿单核细胞存在可纠正的成熟缺陷。淋巴因子和重组IFN-α对DR抗原表达的诱导性表明它们在免疫反应调节中起重要作用。
