Wilson A P
J Natl Cancer Inst. 1984 Mar;72(3):513-21.
An established cell line derived from the ascites of a patient with serous cystadenocarcinoma of the ovary has been characterized. Features studied included morphology, ultrastructure, clonogenicity in soft agar, population doubling time, karyotype, and chemosensitivity. The results indicated that the cells growing in culture were malignant ovarian tumor cells. These cells retained the ability to form free-floating cysts in culture, which were also present in the original ascitic fluid. The cell line had a chromosome number of 80-92 with no distinct mode and 15 stable markers. At passage 4 the cell line showed resistance to doxorubicin [adriamycin (ADM)], phosphoramide mustard (PM), and cisplatin [cis-dichlorodiammineplatinum(II)] (CIS) but rapidly reverted to CIS sensitivity. At passage 25 the cell line was still resistant to ADM and PM, but by passage 59 sensitivity to these drugs appeared to have increased. Frozen cells from passage 15 onward are available.
已对源自一名卵巢浆液性囊腺癌患者腹水的既定细胞系进行了特性描述。所研究的特征包括形态学、超微结构、软琼脂中的克隆形成能力、群体倍增时间、核型和化学敏感性。结果表明，培养中生长的细胞是恶性卵巢肿瘤细胞。这些细胞在培养中保留了形成游离漂浮囊肿的能力，原始腹水中也存在这种囊肿。该细胞系的染色体数为80 - 92条，无明显众数，有15个稳定标记。在第4代时，该细胞系对阿霉素[ Adriamycin (ADM)]、磷酰胺氮芥(PM)和顺铂[顺二氯二氨合铂(II)] (CIS)表现出抗性，但迅速恢复对CIS的敏感性。在第25代时，该细胞系仍对ADM和PM有抗性，但到第59代时，对这些药物的敏感性似乎有所增加。从第15代起的冻存细胞可供使用。
