Marcus A J, Safier L B, Ullman H L, Broekman M J, Islam N, Oglesby T D, Gorman R R
Proc Natl Acad Sci U S A. 1984 Feb;81(3):903-7. doi: 10.1073/pnas.81.3.903.
A new metabolite of arachidonic acid, formed during interaction between thrombin- or collagen-stimulated platelets and unstimulated neutrophils, has been demonstrated by both thin-layer radiochromatography and high-performance liquid chromatography. Production of the 3H-labeled metabolite in combined suspensions containing [3H]arachidonate-labeled platelets and unlabeled neutrophils from aspirin-treated donors suggested that platelet 3H-labeled 12S-hydroxy-5,8-cis,10-trans,14-cis-icosatetraenoic acid (12-HETE) was the precursor. This was confirmed by identification of the same product when purified 12-[3H]HETE was added directly to unstimulated neutrophils. Hydrogenation and oxidation of the isolated product, followed by gas chromatography-mass spectrometry showed the structure to be 12S,20-dihydroxyicosatetraenoic acid. These experiments further show that platelet stimuli known to occur in vivo may initiate metabolic interactions between different cell types via the arachidonic acid pathway.
通过薄层层析放射色谱法和高效液相色谱法已证实，在凝血酶或胶原刺激的血小板与未刺激的中性粒细胞相互作用过程中会形成一种新的花生四烯酸代谢物。在含有[³H]花生四烯酸盐标记的血小板和来自阿司匹林治疗供体的未标记中性粒细胞的混合悬浮液中产生³H标记的代谢物，这表明血小板的³H标记的12S-羟基-5,8-顺式，10-反式，14-顺式-二十碳四烯酸（12-HETE）是前体。当将纯化的12-[³H]HETE直接添加到未刺激的中性粒细胞中时，鉴定出相同的产物，从而证实了这一点。对分离出的产物进行氢化和氧化，然后进行气相色谱-质谱分析，结果表明其结构为12S,20-二羟基二十碳四烯酸。这些实验进一步表明，已知在体内发生的血小板刺激可能通过花生四烯酸途径引发不同细胞类型之间的代谢相互作用。
