Talcott R E, King J
J Natl Cancer Inst. 1984 May;72(5):1113-6.
A widely used pesticide, 1,3-dichloropropene [(DCP) CAS: 542-75-6], has been reported to be mutagenic to Salmonella typhimurium TA100, but large variations in specific mutagenic activity have been observed among different preparations. The purposes of this investigation were to determine the probable cause of the interpreparational variation and to provide new information on the nature of the mutagenic activity. Four preparations were assayed for mutagenic activity before and after silicic acid chromatography. None of the preparations retained mutagenic activity after chromatography, but each contained direct-acting mutagenic polar impurities. The specific mutagenic activities of the unpurified DCP samples appeared to be determined by the mutagenic activities of their polar impurities. A mixture of mutagenic polar impurities could be regenerated by refluxing a purified DCP preparation for 6 hours. The fraction of polar impurities from one of the preparations was analyzed by gas chromatography-mass spectroscopy. Although its composition was too complex to characterize completely, two known mutagens, epichlorhydrin (CAS: 106-89-8; 1-chloro-2,3-epoxypropane) and 1,3-dichloro-2-propanol (CAS: 96-23-1), were tentatively identified. In view of these results, future studies are required to establish whether DCP itself is a chemical carcinogen or whether its previously observed carcinogenicity resulted from the presence of mutagenic impurities.
一种广泛使用的农药，1,3 - 二氯丙烯[（DCP），化学物质登记号：542 - 75 - 6]，据报道对鼠伤寒沙门氏菌TA100具有致突变性，但在不同制剂中观察到特定致突变活性存在很大差异。本研究的目的是确定制剂间差异的可能原因，并提供有关致突变活性本质的新信息。对四种制剂在硅酸色谱分离前后的致突变活性进行了测定。色谱分离后，所有制剂均未保留致突变活性，但每种制剂都含有直接作用的致突变性极性杂质。未纯化的DCP样品的特定致突变活性似乎由其极性杂质的致突变活性决定。通过将纯化的DCP制剂回流6小时，可以再生出致突变性极性杂质的混合物。用气相色谱 - 质谱联用仪分析了其中一种制剂的极性杂质部分。尽管其组成过于复杂，无法完全表征，但初步鉴定出两种已知的诱变剂，环氧氯丙烷（化学物质登记号：106 - 89 - 8；1 - 氯 - 2,3 - 环氧丙烷）和1,3 - 二氯 - 2 - 丙醇（化学物质登记号：96 - 23 - 1）。鉴于这些结果，需要进一步研究以确定DCP本身是否为化学致癌物，或者其先前观察到的致癌性是否源于诱变杂质的存在。
