Donahue E V, McCann J, Ames B N
Cancer Res. 1978 Feb;38(2):431-8.
We have used high-pressure liquid chromatography and the Salmonella/microsome mutagenicity test to look for mutagenic impurities in 11 carcinogens and noncarcinogens. Because of the million-fold range in mutagenic potency observed in the Salmonella test, even trace amounts of potent mutagenic impurities in a nonmutagenic compound could be detected. The mutagenicity of 7-hydroxy-2-acetylaminofluorene, a noncarcinogen in the standard animal carcinogenicity tests, is shown to be due to a small amount of impurity, which is probably the potent carcinogen 2-acetylaminofluorene. This is discussed in relation to the statistical limitations of animal carcinogenicity tests. We also discuss the role of mitogenic impurities in assessing the mutagenicity of environmental (and industrial) chemicals with high-sensitivity mutagenicity assays, such as the Salmonella/microsome test.
我们运用高压液相色谱法和沙门氏菌/微粒体诱变性试验，来探寻11种致癌物和非致癌物中的致突变杂质。鉴于在沙门氏菌试验中观察到诱变性效力存在百万倍的差异，即便非诱变化合物中存在痕量的强效诱变杂质也能够被检测出来。在标准动物致癌性试验中为非致癌物的7-羟基-2-乙酰氨基芴的诱变性，被证明是由少量杂质所致，该杂质可能是强效致癌物2-乙酰氨基芴。结合动物致癌性试验的统计学局限性对此进行了讨论。我们还讨论了促有丝分裂杂质在通过高灵敏度诱变性测定法（如沙门氏菌/微粒体试验）评估环境（和工业）化学品诱变性中的作用。
