Inada T, Mims C A
Nature. 1984;309(5963):59-61. doi: 10.1038/309059a0.
Infection of mice with lactate dehydrogenase virus (LDV) leads to elevation of plasma lactate dehydrogenase, lifelong viraemia and perturbations of cell-mediated and humoral immune responses. The virus replicates exclusively in a restricted set of macrophages, but the basis for restricted cell susceptibility is unknown. By immunofluorescence techniques we have found that the per cent infected was the same as the per cent expressing antigens encoded by the I region of the major histocompatibility complex (Ia). Infection of CBA strain I-A+ peritoneal macrophages was blocked when cells were treated simultaneously with monoclonal antibody to I-A and I-E, but not with either antibody separately. LDV infectivity was inactivated when virus was treated with purified rat glycoprotein homologous to mouse I-A and I-E antigens. These results indicate that the receptors for LDV are I-A and I-E antigens. Selective infection of Ia-positive macrophages may have an important effect on the immunological capability of infected mice.
用乳酸脱氢酶病毒（LDV）感染小鼠会导致血浆乳酸脱氢酶升高、终身病毒血症以及细胞介导和体液免疫反应紊乱。该病毒仅在一组有限的巨噬细胞中复制，但细胞易感性受限的基础尚不清楚。通过免疫荧光技术，我们发现被感染的百分比与表达主要组织相容性复合体（Ia）I区编码抗原的百分比相同。当用抗I-A和I-E的单克隆抗体同时处理细胞时，CBA品系I-A+腹膜巨噬细胞的感染被阻断，但单独使用任何一种抗体时则不会。当用与小鼠I-A和I-E抗原同源的纯化大鼠糖蛋白处理病毒时，LDV的感染性被灭活。这些结果表明，LDV的受体是I-A和I-E抗原。Ia阳性巨噬细胞的选择性感染可能对受感染小鼠的免疫能力产生重要影响。
