Nickerson J M, Piatigorsky J
Proc Natl Acad Sci U S A. 1984 May;81(9):2611-5. doi: 10.1073/pnas.81.9.2611.
A full-length chicken delta-crystallin cDNA (p delta Cr17) was cloned and subjected to sequence analysis. The cDNA was shown to be full-length by both primer extension and S1 and mung bean nuclease experiments. Thus, the complete amino acid sequence of delta-crystallin is now available. The delta-crystallin polypeptide has a molecular mass of 48,542 daltons, as expected from its behavior on NaDodSO4/polyacrylamide gels. delta-Crystallin has one tryptophan, no cysteines, a prevalence of leucines (15%), and a paucity of aromatic residues. High alpha-helical content throughout the protein was predicted from the amino acid sequence. Nucleic acid sequence analysis suggests that delta-crystallin gene 1 encodes the mRNA that gave rise to the cDNA clone. These data provide a basis for the detailed analysis of the two delta-crystallin genes.
克隆了一个全长鸡δ-晶体蛋白cDNA（pδCr17）并进行了序列分析。通过引物延伸以及S1和绿豆核酸酶实验证明该cDNA是全长的。因此，现在可以获得δ-晶体蛋白完整的氨基酸序列。δ-晶体蛋白多肽的分子量为48,542道尔顿，这与其在十二烷基硫酸钠/聚丙烯酰胺凝胶上的行为预期一致。δ-晶体蛋白有一个色氨酸，没有半胱氨酸，亮氨酸含量较高（15%），芳香族残基较少。根据氨基酸序列预测整个蛋白质具有较高的α-螺旋含量。核酸序列分析表明，δ-晶体蛋白基因1编码产生该cDNA克隆的mRNA。这些数据为详细分析两个δ-晶体蛋白基因提供了基础。
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