Yasuda K, Nakajima N, Isobe T, Okada T S, Shimura Y
EMBO J. 1984 Jun;3(6):1397-402. doi: 10.1002/j.1460-2075.1984.tb01983.x.
The nucleotide sequence of a full length cDNA of delta-crystallin mRNA from chicken lens has been determined using a delta-crystallin cDNA clone (pB delta 11), which represents the mRNA sequence of 1530 nucleotides from the poly(A) junction but does not contain the 5'-terminal sequence of 44 nucleotides of the mRNA. The 5'-terminal sequence of the mRNA, absent in the cDNA clone, has been determined with a stretch of cDNA sequence by the primer extension procedure. The amino acid sequence deduced from the nucleotide sequence is consistent with the amino acid sequences of several tryptic peptides, the total amino acid composition, and the mol. wt. of delta-crystallin estimated by SDS-polyacrylamide gel electrophoresis. The computer-assisted analysis predicts high alpha-helical content throughout the polypeptide. Sequence analyses have revealed that gene 1 encodes the mRNA from which the cDNA clone was derived.
利用一个δ-晶体蛋白cDNA克隆（pBδ11）测定了来自鸡晶状体的δ-晶体蛋白mRNA全长cDNA的核苷酸序列，该克隆代表了从多聚腺苷酸连接点起1530个核苷酸的mRNA序列，但不包含mRNA 5′端44个核苷酸的序列。通过引物延伸法，利用一段cDNA序列确定了cDNA克隆中缺失的mRNA 5′端序列。从核苷酸序列推导的氨基酸序列与几个胰蛋白酶肽段的氨基酸序列、总氨基酸组成以及通过SDS-聚丙烯酰胺凝胶电泳估计的δ-晶体蛋白的分子量一致。计算机辅助分析预测整个多肽具有高α-螺旋含量。序列分析表明，基因1编码了产生该cDNA克隆的mRNA。
