鸡晶状体晶状体蛋白DNA序列显示至少有两个δ-晶状体蛋白基因。

Chicken lens crystallin DNA sequences show at least two delta-crystallin genes.

作者信息

Bhat S P, Jones R E, Sullivan M A, Piatigorsky J

出版信息

Nature. 1980 Mar 20;284(5753):234-8. doi: 10.1038/284234a0.

Abstract

Restriction analysis of chicken DNA using probes derived from a cloned cDNA and analysis of cloned genomic DNA fragments containing delta-crystallin gene sequences have indicated the presence of at least two non-allelic genes for delta-crystallin, the first and principal crystallin synthesised in the embryonic chicken lens. Electron microscopic analyses of three cloned genomic fragments revealed that the delta-crystallin mRNA gene sequences are interrupted at least 14 times in one of the delta-crystallin genes.

摘要

利用从克隆的互补DNA（cDNA）衍生而来的探针进行鸡DNA的限制性分析，以及对包含δ-晶体蛋白基因序列的克隆基因组DNA片段的分析表明，至少存在两个非等位的δ-晶体蛋白基因，δ-晶体蛋白是胚胎鸡晶状体中合成的第一种也是主要的晶体蛋白。对三个克隆的基因组片段进行电子显微镜分析发现，在其中一个δ-晶体蛋白基因中，δ-晶体蛋白信使核糖核酸（mRNA）基因序列至少被中断了14次。