Mahdavi V, Chambers A P, Nadal-Ginard B
Proc Natl Acad Sci U S A. 1984 May;81(9):2626-30. doi: 10.1073/pnas.81.9.2626.
Two ventricular myosin heavy chains (MHCs), alpha and beta, which exhibit different levels of ATPase activity, are differentially expressed during development, in response to thyroid hormone and in several pathological conditions. We have isolated and analyzed the structure of the genes coding for alpha- and beta-MHC mRNAs in the rat. Detailed analysis of eight overlapping MHC genomic clones shows that the alpha- and beta-MHC genes are organized in tandem and span 50 kilobases of the chromosome. The beta-MHC gene, predominantly expressed in late fetal life, is located 4 kilobases upstream from the alpha-MHC gene, predominantly expressed in the adult. These two genes are very closely related at the nucleotide sequence level, suggesting that they have arisen by duplication of a common ancestor, yet their expression in the ventricular myocardium has been shown to be regulated in an antithetic fashion by thyroid hormone.
两种心室肌球蛋白重链（MHCs），α和β，具有不同水平的ATP酶活性，在发育过程中、对甲状腺激素的反应以及几种病理状态下呈差异表达。我们已经分离并分析了大鼠中编码α-和β-MHC mRNA的基因结构。对八个重叠的MHC基因组克隆的详细分析表明，α-和β-MHC基因串联排列，跨越染色体的50千碱基。主要在胎儿后期表达的β-MHC基因位于主要在成体中表达的α-MHC基因上游4千碱基处。这两个基因在核苷酸序列水平上非常密切相关，表明它们是由一个共同祖先基因复制而来，但它们在心室心肌中的表达已被证明受甲状腺激素以相反的方式调节。
