Björklund A, Stenevi U, Schmidt R H, Dunnett S B, Gage F H
Acta Physiol Scand Suppl. 1983;522:9-18.
Dissociated dopamine-rich cell suspensions were prepared from the ventral mesencephalon of rat embryos and injected in one or several sites in striatal and non-striatal regions in the dopaminergically denervated brain of adult rats. While the grafts survived well in all sites, the dopamine fibre outgrowth was markedly different depending on whether the grafts occurred in an area normally innervated by the mesencephalic dopamine neurones (i.e. neostriatum or nc. accumbens) or in areas not normally innervated by these neurones (i.e. parietal cortex, lateral hypothalamus or substantia nigra). Moreover, in grafts placed at different sites along the trajectory of the nigrostriatal pathway the outgrowing fibres remained confined to the graft, and there was little evidence that the implanted neurones could elongate their axons along the pathway of the nigrostriatal tract to reach the striatum from a distance. Thus, the intracerebral suspension grafts provided efficient reinnervation of a denervated target only when placed in the immediate vicinity of the target area. The results of multiple graft placements indicate that a relatively complete restoration of a lost innervation should be possible to achieve in large areas of the brain, such as the striatal complex, with the suspension grafting technique.
从大鼠胚胎的腹侧中脑制备富含多巴胺的解离细胞悬液，并将其注射到成年大鼠多巴胺能去神经支配脑的纹状体和非纹状体区域的一个或多个部位。虽然移植物在所有部位都存活良好，但多巴胺纤维的生长明显不同，这取决于移植物是发生在通常由中脑多巴胺神经元支配的区域（即新纹状体或伏隔核）还是在通常不由这些神经元支配的区域（即顶叶皮质、外侧下丘脑或黑质）。此外，在沿着黑质纹状体通路轨迹的不同部位植入的移植物中，生长出的纤维仍局限于移植物内，几乎没有证据表明植入的神经元能够沿着黑质纹状体束的通路延伸其轴突，从远处到达纹状体。因此，脑内细胞悬液移植物只有放置在靶区域的紧邻位置时，才能有效地重新支配去神经的靶组织。多次移植物植入的结果表明，使用细胞悬液移植技术，在大脑的大面积区域，如纹状体复合体，实现相对完全的失神经支配恢复应该是可能的。
