Dunnett S B, Björklund A, Schmidt R H, Stenevi U, Iversen S D
Acta Physiol Scand Suppl. 1983;522:29-37.
Single and multiple implants of nigral cell suspensions were grafted to the forebrains of rats with unilateral 6-hydroxydopamine-induced dopamine denervations. Control lesions alone induced a marked behavioural asymmetry, as assessed by amphetamine- and apomorphine-induced rotation, sensorimotor tests and side bias in an unbaited T-maze, and the animals were hyperactive to a low dose of apomorphine. Single suspension placements into different denervated striatal regions were capable of reversing the behavioural asymmetries dependent upon the specific placement for each test. Multiple suspension grafts were capable of reversing all behavioural asymmetries, and additionally abolished the supersensitive hyperactivity to apomorphine. By contrast, single suspension grafts placed into the substantia nigra or lateral hypothalamus had no detectable effect on any functional measure. The results indicate that nigral suspension grafts can be at least as effective as solid grafts in reversing the functional deficits induced by dopamine denervation, provided that placements are selected within appropriate dopamine terminal regions of the forebrain (e.g. caudate-putamen or nucleus accumbens).
将单个和多个黑质细胞悬液植入单侧6-羟基多巴胺诱导的多巴胺去神经支配的大鼠前脑。单独的对照损伤会导致明显的行为不对称，通过苯丙胺和阿扑吗啡诱导的旋转、感觉运动测试以及在无诱饵T迷宫中的侧偏来评估，并且动物对低剂量阿扑吗啡表现出多动。将单个悬液植入不同的去神经支配纹状体区域能够根据每个测试的特定植入部位逆转行为不对称。多个悬液移植能够逆转所有行为不对称，并且还消除了对阿扑吗啡的超敏多动。相比之下，将单个悬液移植到黑质或下丘脑外侧对任何功能指标均无明显影响。结果表明，只要在前脑适当的多巴胺终末区域（如尾状核-壳核或伏隔核）进行植入，黑质悬液移植在逆转多巴胺去神经支配引起的功能缺陷方面至少与实体移植一样有效。
