Morisawa K
Nihon Seikeigeka Gakkai Zasshi. 1983 Dec;57(12):1937-50.
The acid phosphatase in the cells of bone and soft parts tumors, and of other tumorous conditions in our Department of Orthopedic Surgery in Kumamoto University Medical School from mid-1979 through mid-1983 were analysed by light microscopic and electron microscopic histochemical studies and their inhibition studies. The histochemical and their inhibiting studies of acid phosphatase by azo dye method in the cells of bone and soft parts tumors and of other tumorous conditions were undertaken in order to characterize them with a view to providing helpful diagnostic features. The acid phosphatase in some giant cells and tumor cells of several kinds of tumors, whose reaction against inhibitors was different from that of lysosomal acid phosphatase, was observed. In the giant cells of giant cell tumor of bone, acid-para-nitrophenyl phosphatase was demonstrated by the method of Miyayama , et al. using sodium-para-nitrophenyl phosphate as a substrate. In addition, the fine structural localization of acid phosphatase in giant cell tumor of bone was studied by Gomori's method and by the method of Miyayama , et al. By Gomori's method, acid phosphatase activity was demonstrated in lysosome, secondary lysosome-like organelles and the digestive vacuoles in the giant cells. In the stromal cells, that activity was demonstrated in lysosomes. By the method of Miyayama , et al., acid para-nitrophenyl phosphatase was demonstrated in the Golgi complex and the cisternae of the rough endoplasmic reticulum in the giant cell. Therefore, in the giant cells and the tumor cells of some kinds of tumors, non-lysosomal acid phosphatase besides lysosomal acid phosphatase was recognized. The demonstration of non-lysosomal acid phosphatase was a useful tool for the differential diagnosis of tumors and tumorous conditions in bone and soft parts.
1979年年中至1983年年中，我们对熊本大学医学院骨科的骨与软组织肿瘤细胞以及其他肿瘤性疾病中的酸性磷酸酶进行了光镜和电镜组织化学研究及其抑制研究。为了对骨与软组织肿瘤细胞以及其他肿瘤性疾病进行特征描述，以便提供有用的诊断特征，我们采用偶氮染料法对酸性磷酸酶进行了组织化学及其抑制研究。观察到几种肿瘤的一些巨细胞和肿瘤细胞中的酸性磷酸酶，其对抑制剂的反应与溶酶体酸性磷酸酶不同。在骨巨细胞瘤的巨细胞中，采用宫山等人的方法，以对硝基苯磷酸钠为底物，证明了酸性对硝基苯磷酸酶的存在。此外，还采用Gomori法和宫山等人的方法研究了骨巨细胞瘤中酸性磷酸酶的精细结构定位。通过Gomori法，在巨细胞的溶酶体、次级溶酶体样细胞器和消化泡中显示出酸性磷酸酶活性。在基质细胞中，该活性在溶酶体中显示。通过宫山等人的方法，在巨细胞的高尔基体和粗面内质网池中证明了酸性对硝基苯磷酸酶的存在。因此，在某些肿瘤的巨细胞和肿瘤细胞中，除了溶酶体酸性磷酸酶外，还识别出了非溶酶体酸性磷酸酶。非溶酶体酸性磷酸酶的显示是骨与软组织肿瘤及肿瘤性疾病鉴别诊断的有用工具。
