McCarthy E F, Serrano J A, Wasserkrug H L, Dorfman H D
Clin Orthop Relat Res. 1979 Jun(141):295-302.
Three cases of giant cell tumor of bone were studied with the light and electron microscopes to determine the histochemical and cytochemical distribution of acid phosphatase isoenzymes. Using beta-glycerophosphate as a nonspecific substrate, acid phosphatase was found in the giant cells as well as the stromal cells. Ultrastructurally, using this substrate, the enzyme was found to be associated with lysosomes in the stromal cells and giant cells and also with the profuse system of tubules and vesicles in the cytoplasm of the giant cells. Using phosphorylcholine and D-ephedrinephosphate, which are substrates for a specific secretory isoenzyme of acid phosphatase, activity was found only in the vesicles of the giant cell cytoplasm. Lysosomes did not show activity with these substrates. Multinucleated cells of giant cell tumor contain a specific secretory type of acid phosphatase which is not present in the stromal cells. This suggests that the giant cells are attempting to form a secretory system similar to osteoclasts and that the vesicles in the cytoplasm which contain this acid phosphates are the morphologic expression of an abortive secretory system.
对三例骨巨细胞瘤进行了光镜和电镜研究，以确定酸性磷酸酶同工酶的组织化学和细胞化学分布。以β-甘油磷酸作为非特异性底物，在巨细胞和基质细胞中均发现了酸性磷酸酶。超微结构上，使用该底物时，发现该酶与基质细胞和巨细胞中的溶酶体相关，也与巨细胞质中丰富的小管和小泡系统相关。使用酸性磷酸酶特异性分泌同工酶的底物磷酸胆碱和D-麻黄碱磷酸盐时，仅在巨细胞质的小泡中发现活性。溶酶体对这些底物无活性。骨巨细胞瘤的多核细胞含有一种基质细胞中不存在的特异性分泌型酸性磷酸酶。这表明巨细胞正试图形成类似于破骨细胞的分泌系统，且细胞质中含有这种酸性磷酸酶的小泡是流产分泌系统的形态学表现。
