戴尔-艾辛格分析在收缩系统邻近映射中的应用。
Application of the Dale-Eisinger analysis to proximity mapping in the contractile system.
作者信息
Torgerson P M, Morales M F
出版信息
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3723-7. doi: 10.1073/pnas.81.12.3723.
Abstract
Fluorescence anisotropy decays were used to quantify the degree of rapid librational motion associated with several fluorescent probes attached to contractile proteins. This information allows an analysis of how far kappa 2, the resonance energy transfer orientation factor, may deviate from the dynamically averaged value of 2/3. Extrema can then be set on R0, the critical transfer distance, and hence on the interprobe distance. These results set maximum ranges on possible distances between several probe pairs used in the mapping of contractile protein structure by resonance energy transfer.
摘要
荧光各向异性衰减被用于量化与附着在收缩蛋白上的几种荧光探针相关的快速摆动运动程度。该信息有助于分析κ2（共振能量转移取向因子）可能偏离动态平均值2/3的程度。然后可以在临界转移距离R0上设定极值，进而在探针间距离上设定极值。这些结果为通过共振能量转移绘制收缩蛋白结构时使用的几对探针之间的可能距离设定了最大范围。
相似文献
1
Application of the Dale-Eisinger analysis to proximity mapping in the contractile system.戴尔-艾辛格分析在收缩系统邻近映射中的应用。
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3723-7. doi: 10.1073/pnas.81.12.3723.
2
Orientation factor in steady-state and time-resolved resonance energy transfer measurements.稳态和时间分辨共振能量转移测量中的取向因子。
Biochemistry. 1992 Sep 1;31(34):7939-47. doi: 10.1021/bi00149a027.
3
Fluorescence resonance energy transfer measurements of distances in actin and myosin. A critical evaluation.肌动蛋白和肌球蛋白中距离的荧光共振能量转移测量：批判性评估
J Muscle Res Cell Motil. 1987 Apr;8(2):97-117. doi: 10.1007/BF01753986.
4
Interhead distances in myosin attached to F-actin estimated by fluorescence energy transfer spectroscopy.通过荧光能量转移光谱法估算附着于F-肌动蛋白的肌球蛋白中的头部间距离。
Biophys J. 1997 Aug;73(2):895-904. doi: 10.1016/S0006-3495(97)78122-0.
5
Fluorescence anisotropy imaging microscopy for homo-FRET in living cells.用于活细胞中同源荧光共振能量转移的荧光各向异性成像显微镜技术。
Methods Cell Biol. 2008;85:395-414. doi: 10.1016/S0091-679X(08)85017-0.
6
Ultrafast energy relaxation and anisotropy decay of the librational motion in liquid water: A molecular dynamics study.超快能量弛豫和液体水中振动运动的各向异性衰减：分子动力学研究。
J Chem Phys. 2009 Oct 28;131(16):164511. doi: 10.1063/1.3254518.
7
Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment.I类主要组织相容性复合体分子中的序列内绿色荧光蛋白，用于膜环境荧光各向异性测量的刚性探针。
Biophys J. 2003 Jun;84(6):4078-86. doi: 10.1016/S0006-3495(03)75133-9.
8
Fluorescence resonance energy transfer spectroscopy is a reliable "ruler" for measuring structural changes in proteins. Dispelling the problem of the unknown orientation factor.荧光共振能量转移光谱是用于测量蛋白质结构变化的可靠“尺子”。消除了取向因子未知的问题。
J Struct Biol. 1995 Sep-Oct;115(2):175-85. doi: 10.1006/jsbi.1995.1042.
9
Perrin and Förster unified: Dual-laser triple-polarization FRET (3polFRET) for interactions at the Förster-distance and beyond.佩兰和福斯特提出统一的双激光三偏振荧光共振能量转移技术（3polFRET）用于研究在福斯特距离及以外的相互作用。
Biochim Biophys Acta. 2016 Apr;1863(4):703-16. doi: 10.1016/j.bbamcr.2016.02.002. Epub 2016 Feb 6.
10
A conformational change in F-actin when myosin binds: fluorescence resonance energy transfer detects an increase in the radial coordinate of Cys-374.肌球蛋白结合时F-肌动蛋白的构象变化：荧光共振能量转移检测到半胱氨酸-374径向坐标增加。
Biochemistry. 1997 Jun 17;36(24):7353-60. doi: 10.1021/bi962588l.
引用本文的文献
1
The use of the isotropic orientation factor in fluorescence resonance energy transfer (FRET) studies of the actin filament.各向同性取向因子在肌动蛋白丝的荧光共振能量转移（FRET）研究中的应用。
J Fluoresc. 1992 Sep;2(3):141-55. doi: 10.1007/BF00866929.
2
On the origin and transmission of force in actomyosin subfragment 1.关于肌动球蛋白亚片段1中力的起源与传递
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2204-8. doi: 10.1073/pnas.86.7.2204.
3
Resonance energy transfer from a cylindrical distribution of donors to a plane of acceptors. Location of apo-B100 protein on the human low-density lipoprotein particle.从供体的圆柱形分布到受体平面的共振能量转移。载脂蛋白B100蛋白在人低密度脂蛋白颗粒上的定位。
Biophys J. 1990 Sep;58(3):665-75. doi: 10.1016/S0006-3495(90)82409-7.
本文引用的文献
1
Fluorescence energy transfer studies of skeletal troponin C proximity between methionine-25 and cysteine-98.骨骼肌肌钙蛋白C中甲硫氨酸-25与半胱氨酸-98之间接近程度的荧光能量转移研究。
Biochemistry. 1982 Oct 12;21(21):5135-42. doi: 10.1021/bi00264a005.
2
Investigation of reduced nicotinamide adenine dinucleotide phosphate and acyl-binding sites on avian fatty acid synthase.禽脂肪酸合酶上还原型烟酰胺腺嘌呤二核苷酸磷酸及酰基结合位点的研究
Biochemistry. 1982 Jun 8;21(12):2863-70. doi: 10.1021/bi00541a009.
3
Time-resolved fluorescence of the two tryptophans in horse liver alcohol dehydrogenase.马肝醇脱氢酶中两个色氨酸的时间分辨荧光
Biochemistry. 1981 Jul 21;20(15):4369-77. doi: 10.1021/bi00518a021.
4
On the mechanism of energy transduction in myosin subfragment 1.关于肌球蛋白亚片段1中能量转换的机制。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2060-4. doi: 10.1073/pnas.81.7.2060.
5
Fluorescence energey transfer in myosin subfragment-1.肌球蛋白亚片段-1中的荧光能量转移
Biochemistry. 1980 Feb 19;19(4):774-84. doi: 10.1021/bi00545a025.
6
Spatial relationship between a fast-reacting thiol and a reactive lysine residue of myosin subfragment 1.肌球蛋白亚片段1的快速反应硫醇与反应性赖氨酸残基之间的空间关系。
Biochemistry. 1982 Oct 26;21(22):5661-8. doi: 10.1021/bi00265a042.
7
On the molecular weight of myosin. II.关于肌球蛋白的分子量。二。
Biochemistry. 1966 Feb;5(2):515-21. doi: 10.1021/bi00866a017.
8
On the analysis of fluorescence decay kinetics by the method of least-squares.通过最小二乘法对荧光衰减动力学进行分析。
Anal Biochem. 1974 Jun;59(2):583-98. doi: 10.1016/0003-2697(74)90312-1.
9
Synthesis and characterization of two fluorescent sulfhydryl reagents.两种荧光巯基试剂的合成与表征
Biochemistry. 1973 Oct 9;12(21):4154-61. doi: 10.1021/bi00745a019.
10
The measurement of actin concentration in solution: a comparison of methods.溶液中肌动蛋白浓度的测量：方法比较。
Anal Biochem. 1974 Nov;62(1):66-74. doi: 10.1016/0003-2697(74)90367-4.
Zotero 插件