Danielson K G, Oborn C J, Durban E M, Butel J S, Medina D
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3756-60. doi: 10.1073/pnas.81.12.3756.
An epithelial cell line, designated COMMA-1D, was derived from mammary tissue of BALB/c mice in the middle of pregnancy. This line, in continuous cell culture for 12 months, exhibits several characteristics distinctive of normal mammary epithelial cells, including induction of casein synthesis in vitro and normal duct morphogenesis in the cleared mammary fat pads of syngeneic mice. The cells also form domes in high density culture and are positive for keratin intermediate filaments by indirect immunofluorescence. COMMA-1D cells have a near diploid number of chromosomes and do not grow in suspension culture or produce tumors in syngeneic hosts. This cell line should prove useful for studies examining the regulation of normal cellular differentiation of mammary cells as well as transformation of epithelial cells to the preneoplastic and neoplastic phenotypes.
一种名为COMMA-1D的上皮细胞系，源自妊娠中期BALB/c小鼠的乳腺组织。该细胞系在连续细胞培养12个月后，表现出正常乳腺上皮细胞的几个独特特征，包括体外诱导酪蛋白合成以及在同基因小鼠清除的乳腺脂肪垫中形成正常导管形态。这些细胞在高密度培养时还会形成穹顶，通过间接免疫荧光法检测角蛋白中间丝呈阳性。COMMA-1D细胞的染色体数接近二倍体，在悬浮培养中不生长，也不会在同基因宿主中产生肿瘤。该细胞系对于研究乳腺细胞正常细胞分化的调控以及上皮细胞向肿瘤前和肿瘤表型的转化应具有重要作用。
