Medina D, Oborn C J
Cancer Res. 1980 Nov;40(11):3982-7.
The conditions for growing preneoplastic mammary epithelial cells in primary cell cultures with serum-free factor-defined medium were established in a series of experiments. The cells, grown in Dulbecco's modified Eagle's medium with 0.6 mM Ca2+, epidermal growth factor, transferrin, insulin, and Na2SeO3, were fibroblast free, exhibited saturation densities similar to those of cells grown in serum-containing medium, and actively incorporated [3H]thymidine into DNA, as demonstrated by biochemical and autoradiography assays. The total growth fraction over a seven-day experiment was 77%. Fetuin was sufficient as a serum substitute for attachment of cells to the dish, although it was apparently not necessary for continued growth of the cells. The possible benefits of growing preneoplastic mammary epithelial cells in serum-free factor-defined medium are discussed.
在一系列实验中确定了在无血清因子定义培养基的原代细胞培养物中培养癌前乳腺上皮细胞的条件。这些细胞在含有0.6 mM Ca2+、表皮生长因子、转铁蛋白、胰岛素和亚硒酸钠的杜尔贝科改良伊格尔培养基中生长，无成纤维细胞，表现出与在含血清培养基中生长的细胞相似的饱和密度，并通过生化和放射自显影分析表明能将[3H]胸腺嘧啶核苷积极掺入DNA。在为期七天的实验中，总生长分数为77%。胎球蛋白足以作为血清替代物使细胞附着于培养皿，尽管它显然不是细胞持续生长所必需的。讨论了在无血清因子定义培养基中培养癌前乳腺上皮细胞的可能益处。
