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[人类染色体中呈分散分布的含Alu DNA片段的结构分析]

[Structural analysis of the Alu-containing DNA fragment with disperse distribution in human chromosomes].

作者信息

Zaĭtsev I Z, Gindilis V M, Shapiro Iu A, Iurov Iu B, Iakovlev A G

出版信息

Genetika. 1984 May;20(5):738-45.

PMID:6588015
Abstract

Nucleotide sequencing of two terminal subfragments of a cloned human DNA fragment has been done. The fragment cloned hybridized dispersively along all chromosomes except for Y chromosome and C heterochromatic chromosome regions. Both subfragments sequenced contain Alu repeats, whose structures differ partially from those of accurately determined sequences of human Alu repeats. The results obtained are discussed in respect of possible usage of Alu repeats containing sequences for construction of special polymorphic molecular markers of human chromosomes.

摘要

已对一个克隆的人类DNA片段的两个末端亚片段进行了核苷酸测序。克隆的片段除了Y染色体和C异染色质染色体区域外,沿所有染色体进行分散杂交。测序的两个亚片段均含有Alu重复序列,其结构部分不同于已准确确定的人类Alu重复序列。针对含Alu重复序列的序列在构建人类染色体特殊多态性分子标记中的可能用途,对所得结果进行了讨论。

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