Meiers J C, Schachtele C F
J Dent Res. 1984 Aug;63(8):1051-5. doi: 10.1177/00220345840630080801.
The bacterial counts obtained with a needle-scrape method for collecting plaque samples from human occlusal fissures with incipient caries were compared with the microflora remaining in the fissures as determined with a fissure removal method. Scraping of six fissures with a sterile needle recovered only 18.2% of the total recovered fissure flora. The needle-scrape method failed to detect specific cariogenic bacteria which were present in four of the fissures. Treatment of nine fissures with an antibacterial solution demonstrated that the needle method failed to detect viable bacteria in eight of the fissures which were subsequently shown to contain bacteria by fissure removal. The commonly used needle-scraping method does not appear adequate for studies on the effect of antimicrobial agents on fissure plaque.
将用针刮法从患有早期龋齿的人类咬合面窝沟采集菌斑样本所获得的细菌计数，与用窝沟去除法测定的窝沟中残留的微生物区系进行了比较。用无菌针刮擦六个窝沟，仅回收了总回收窝沟菌群的18.2%。针刮法未能检测到四个窝沟中存在的特定致龋菌。用抗菌溶液处理九个窝沟表明，针刮法未能检测到八个窝沟中的活菌，而随后通过窝沟去除法显示这些窝沟中含有细菌。常用的针刮法似乎不足以用于研究抗菌剂对窝沟菌斑的影响。
