Meiers J C, Wirthlin M R, Shklair I L
J Dent Res. 1982 Mar;61(3):460-4. doi: 10.1177/00220345820610030301.
The fissure sampling technique consisted of removing the fissure using a bur in a high-speed handpiece with water spray and then suctioning the fissural contents and the water spray into a sterile sampling container. Sixty-eight teeth were sampled. Total bacterial counts, total streptococcal counts, and counts of Streptococcus mutans, Streptococcus sanguis, Streptococcus faecalis, Actinomyces viscosus, and lactobacilli were performed on the fissural samples. There was a four-fold increase in the total number of microorganisms recovered from carious (N = 48) compared to non-carious (N = 20) fissures. S. mutans was the only microorganism common to all carious fissures.
裂隙采样技术包括使用高速手机上的车针并喷水去除裂隙，然后将裂隙内容物和喷水吸到无菌采样容器中。共采集了68颗牙齿的样本。对裂隙样本进行了总细菌计数、总链球菌计数以及变形链球菌、血链球菌、粪肠球菌、黏性放线菌和乳酸杆菌的计数。与非龋性（N = 20）裂隙相比，龋性（N = 48）裂隙中回收的微生物总数增加了四倍。变形链球菌是所有龋性裂隙中唯一共有的微生物。
