Kramer P F, Zelante F, Simionato M R
Pediatric Dentistry, School of Dentistry, University of Sao Paulo, Brazil.
Pediatr Dent. 1993 Mar-Apr;15(2):108-12.
The purpose of this study was to evaluate the effect of acid conditioning and occlusal sealant on microbial colonization of pit and fissures submitted to ameloplasty or left intact. Human enamel blocks containing fissures prepared from the occlusal surfaces of unerupted third molars were implanted in occlusal fillings in molars of 12 patients for seven, 30, 60, and 120 days. After seven days of exposure to the oral environment, the pit and fissure blocks were removed and found to be colonized, mainly with gram-positive coccal flora. The acid-etching procedure itself reduced the number of cultivable microorganisms by about 95%. Subsequent application of occlusal sealant caused a gradual decrease of the remaining viable microorganism throughout the experiment. Despite the decrease of 100% after acid etching in most of the fissures submitted to ameloplasty, the occlusal sealant did not lead to a subsequent significant reduction.
本研究的目的是评估酸蚀处理和窝沟封闭剂对接受牙釉质成形术或未处理的完整窝沟中微生物定植的影响。从未萌出第三磨牙的咬合面制备的含裂隙的人牙釉质块被植入12例患者磨牙的咬合充填物中7天、30天、60天和120天。暴露于口腔环境7天后，取出窝沟块，发现有微生物定植，主要是革兰氏阳性球菌菌群。酸蚀过程本身使可培养微生物数量减少了约95%。在整个实验过程中，随后应用窝沟封闭剂使剩余存活微生物数量逐渐减少。尽管在大多数接受牙釉质成形术的裂隙中酸蚀后微生物数量减少了100%，但窝沟封闭剂并未导致随后的显著减少。
