Kubonishi I, Ohtsuki Y, Yoshimoto S, Miyoshi I
Int J Cell Cloning. 1984 Jul;2(4):243-53. doi: 10.1002/stem.5530020404.
Heterotransplantation was performed to study the maturational capacity of a new Ph1 chromosome-positive chronic myelogenous leukemia cell line (KCL-22). Five million KCL-22 cells of undifferentiated blasts were implanted intraperitoneally into 15 immunosuppressed newborn hamsters. Of the 15 hamsters, 10 that survived developed granulocytic tumors 16-61 days after implantation. The tumor cells were composed of a mixed population of undifferentiated blasts, neutrophils, and eosinophils and had a human female karyotype with double Ph1 chromosomes identical to that found in the KCL-22 cell line used for transplantation. These findings indicate that Ph1 chromosome-positive cells can be induced to mature along the neutrophil-eosinophil lineage after growth in the heterologous hosts.
进行异种移植以研究一种新的费城染色体阳性慢性粒细胞白血病细胞系（KCL-22）的成熟能力。将500万个未分化母细胞的KCL-22细胞经腹腔植入15只免疫抑制的新生仓鼠体内。在这15只仓鼠中，存活下来的10只在植入后16 - 61天出现了粒细胞肿瘤。肿瘤细胞由未分化母细胞、中性粒细胞和嗜酸性粒细胞的混合群体组成，并且具有与用于移植的KCL-22细胞系中发现的相同的双费城染色体的人类女性核型。这些发现表明，费城染色体阳性细胞在异种宿主中生长后可被诱导沿着中性粒细胞 - 嗜酸性粒细胞谱系成熟。
