Miyoshi I, Kubonishi I, Uchida H, Hiraki S, Toki H
Blood. 1976 Mar;47(3):355-61.
Peripheral leukocytes from a male patient with chronic myelogenous leukemia in blast crisis were implanted directly into the intraperitoneal cavity of seven newborn hamsters treated with antilymphocyte serum. At sacrifice 17-19 days after implantation, three hamsters were found to have disseminated tumors. The other four hamsters were cannibalized. Chromosome analysis of cells from the enlarged lymph nodes revealed the presence of a human male karyotype. Most metaphase cells were pseudodiploid with the Ph1 chromosome and isochromosome 17, while a few metaphase cells were classically diploid. Identical chromosome constitutions were observed in the original peripheral leukocytes of the patient. The results strongly suggested simultaneous heterotransplantation of Ph1-positive leukemic and normal leukocytes.
将一名处于原始细胞危象的慢性粒细胞白血病男性患者的外周血白细胞直接植入7只经抗淋巴细胞血清处理的新生仓鼠的腹腔内。在植入后17 - 19天处死时，发现3只仓鼠有播散性肿瘤。另外4只仓鼠被同类相食。对肿大淋巴结中的细胞进行染色体分析，发现存在人类男性核型。大多数中期细胞为具有Ph1染色体和17等臂染色体的假二倍体，而少数中期细胞为典型的二倍体。在患者原来的外周血白细胞中观察到相同的染色体组成。结果强烈提示Ph1阳性白血病细胞和正常白细胞同时进行了异种移植。
