McNamara M J, Schmitt J D, Wykle R L, Daniel L W
Biochem Biophys Res Commun. 1984 Jul 31;122(2):824-30. doi: 10.1016/s0006-291x(84)80108-4.
The human promyelocytic leukemia cell line HL-60 can be differentiated to cells resembling either neutrophils or mononuclear phagocytes by a diverse group of stimuli. However, the underlying mechanisms remain unknown. We report that 1-0-hexadecyl-2-acetyl-sn-glycerol inhibits the growth of HL-60 cells and induces differentiation to cells resembling mononuclear phagocytes. HL-60 cultures incubated for 6 days with 1-0-hexadecyl-2-acetyl-sn-glycerol (5 micrograms/ml) demonstrated a ten-fold increase in nonspecific esterase activity, and produced cells with morphological features similar to those of monocytes and macrophages. Higher concentrations of 1-0-hexadecyl-2-acetyl-sn-glycerol significantly inhibited the growth of HL-60 cells and resulted in the virtual absence of cells resembling the original HL-60 line. 1-0-Oleoyl-2-acetyl-rac-glycerol added under the same conditions did not induce cell differentiation or inhibit cell growth.
人早幼粒白血病细胞系HL-60可通过多种刺激分化为类似中性粒细胞或单核吞噬细胞的细胞。然而，其潜在机制仍不清楚。我们报告1-0-十六烷基-2-乙酰基-sn-甘油可抑制HL-60细胞的生长并诱导其分化为类似单核吞噬细胞的细胞。用1-0-十六烷基-2-乙酰基-sn-甘油（5微克/毫升）培养HL-60细胞6天，非特异性酯酶活性增加了10倍，并产生了形态特征类似于单核细胞和巨噬细胞的细胞。更高浓度的1-0-十六烷基-2-乙酰基-sn-甘油显著抑制HL-60细胞的生长，并导致几乎不存在类似原始HL-60细胞系的细胞。在相同条件下添加的1-0-油酰基-2-乙酰基-rac-甘油不会诱导细胞分化或抑制细胞生长。
