Mukherji B, MacAlister T J, Guha A, Gillies C G, Jeffers D C, Slocum S K
J Natl Cancer Inst. 1984 Sep;73(3):583-93.
The establishment of a spontaneously transformed tumorigenic human fibroblast line, VIP-F:T, is described. This line was developed from a primary culture of normal skin from a donor from whom a separate nontransformed fibroblast line, Pen-F2, also was established. The transformed line VIP-F:T exhibited aneuploid karyotype with a marker chromosome, showed anchorage-independent growth, and produced progressively growing tumors with morphologic characteristics of sarcoma in CD-1 (nu/nu) nude mice. The normal fibroblast line Pen-F2 exhibited diploid karyotype, showed no anchorage-independent growth, and produced no tumors in the nude mice. The spontaneously transformed fibroblast line VIP-F:T and its normal counterpart Pen-F2 will be valuable in studies of oncogene expression and in other investigations relevant to neoplasia.
本文描述了一种自发转化的致瘤性人成纤维细胞系VIP-F:T的建立。该细胞系源自一名供体正常皮肤的原代培养物，从同一供体还建立了一个单独的未转化成纤维细胞系Pen-F2。转化后的细胞系VIP-F:T表现出非整倍体核型并带有一条标记染色体，呈现出不依赖贴壁的生长特性，且在CD-1（nu/nu）裸鼠中产生具有肉瘤形态特征的进行性生长肿瘤。正常成纤维细胞系Pen-F2表现出二倍体核型，未显示出不依赖贴壁的生长特性，且在裸鼠中不产生肿瘤。自发转化的成纤维细胞系VIP-F:T及其正常对应物Pen-F2在癌基因表达研究以及与肿瘤形成相关的其他研究中将具有重要价值。
