Clarkson B H, Wefel J S, Miller I
J Dent Res. 1984 Oct;63(10):1186-9. doi: 10.1177/00220345840630100201.
Subsurface enamel lesions and root surface caries-like lesions were consistently produced in vitro using Streptococcus mutans FA1 cultured in thioglycollate broth containing 3.5% w/v dextrose and 2% w/v gelatin. When viewed in polarized light and after imbibition in water, the enamel lesions had a negatively birefringent surface zone and positively birefringent body of the lesion. Those lesions produced after six weeks, after imbibition in quinoline, exhibited a dark zone. The root surface caries-like lesions exhibited a less-radiolucent surface zone above a heavily demineralized body of the lesion. However, no reactionary dentin was seen in the in vitro lesions.
使用在含有3.5%（重量/体积）葡萄糖和2%（重量/体积）明胶的硫代乙醇酸盐肉汤中培养的变形链球菌FA1，在体外持续产生牙釉质表层下病变和类根面龋病变。在偏光下观察以及在水中浸润后，牙釉质病变有一个负双折射表面区和病变的正双折射主体。六周后产生的那些病变，在喹啉中浸润后，呈现出一个暗区。类根面龋病变在病变的高度脱矿主体上方有一个放射性较低的表面区。然而，在体外病变中未观察到反应性牙本质。
