Naccache P H, Molski T F, Volpi M, Sha'afi R I
J Leukoc Biol. 1984 Sep;36(3):333-40. doi: 10.1002/jlb.36.3.333.
We have examined the effects of free fatty acids on the unstimulated and N-formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe) stimulated rabbit neutrophil aggregation and degranulation. As previously reported, arachidonic acid was found to induce by itself an aggregatory response, and in the presence of cytochalasin B, a degranulation response from the neutrophils. The aggregatory property, but not the secretory activity of arachidonic acid, was shared by oleic acid, linoleic acid, linolenic acid, linoelaidic acid, and lauric acid but not by elaidic acid, caproic acid, stearic acid, and arachidic acid. In addition, several free fatty acids (oleic acid, linoleic acid, linolenic acid, arachidonic acid, and linoelaidic acid) were found to inhibit the aggregatory and secretory responses of the neutrophils to the addition of f-Met-Leu-Phe. Caproic acid and lauric acid were found to stimulate to a small extent the aggregatory response of the neutrophils to f-Met-Leu-Phe and to leave unaffected their secretory response. Elaidic acid, stearic acid, and arachidic acid, on the other hand, left both stimulated responses unaffected. These results demonstrate that at least two neutrophil functions can be modulated (both positively and negatively) by free fatty acids.
我们研究了游离脂肪酸对未受刺激的以及经N-甲酰甲硫氨酰亮氨酰苯丙氨酸（f-Met-Leu-Phe）刺激的兔中性粒细胞聚集和脱颗粒的影响。如先前报道，发现花生四烯酸自身可诱导聚集反应，并且在细胞松弛素B存在的情况下，可诱导中性粒细胞发生脱颗粒反应。油酸、亚油酸、亚麻酸、反式油酸和月桂酸具有与花生四烯酸相同的聚集特性，但不具有其分泌活性，而反油酸、己酸、硬脂酸和花生酸则不具有该聚集特性。此外，发现几种游离脂肪酸（油酸、亚油酸、亚麻酸、花生四烯酸和反式油酸）可抑制中性粒细胞对添加f-Met-Leu-Phe的聚集和分泌反应。发现己酸和月桂酸在一定程度上可刺激中性粒细胞对f-Met-Leu-Phe的聚集反应，而对其分泌反应无影响。另一方面，反油酸、硬脂酸和花生酸对两种刺激反应均无影响。这些结果表明，游离脂肪酸至少可对中性粒细胞的两种功能产生调节作用（包括正向和负向）。
