O'Flaherty J T, Showell H J, Becker E L, Ward P A
Am J Pathol. 1979 May;95(2):433-44.
In response to aggregating and degranulating stimuli, platelets metabolize endogenous arachidonic acid to bioactive derivatives. These derivatives can stimulate platelets to degranulate and aggregate and, therefore, may be mediators of the platelet response. Because exogenous arachidonic acid also stimulates platelets to degranulate, aggregate, and form these mediators, we examined the effect of adding arachidonic acid to purified human neutrophil suspensions. Micromolar concentrations of arachidonic acid stimulated neutrophils to aggregate but not to degranulate. Cytochalasin B, a potentiator of neutrophil responses to chemotactic factors, also potentiated the arachidonic-acid-induced aggregation response; 5,8,11,14-eicosatetraynoic acid, an inhibitor of arachidonic acid metabolism, blocked this response. Aggregation of neutrophils, was not stimulated by several fatty acids with structural similarity to arachidonic acid. These results suggest that metabolic derivatives of arachidonic acid may be active in stimulating certain neutrophil responses. The role of these derivatives in mediating neutrophil responses to various stimuli needs to be examined.
作为对聚集和脱颗粒刺激的反应，血小板将内源性花生四烯酸代谢为生物活性衍生物。这些衍生物可刺激血小板脱颗粒和聚集，因此可能是血小板反应的介质。由于外源性花生四烯酸也能刺激血小板脱颗粒、聚集并形成这些介质，我们研究了向纯化的人中性粒细胞悬液中添加花生四烯酸的效果。微摩尔浓度的花生四烯酸刺激中性粒细胞聚集，但不刺激其脱颗粒。细胞松弛素B是中性粒细胞对趋化因子反应的增强剂，它也增强了花生四烯酸诱导的聚集反应；5,8,11,14-二十碳四炔酸是花生四烯酸代谢的抑制剂，可阻断这一反应。几种与花生四烯酸结构相似的脂肪酸未刺激中性粒细胞聚集。这些结果表明，花生四烯酸的代谢衍生物可能在刺激某些中性粒细胞反应中具有活性。这些衍生物在介导中性粒细胞对各种刺激的反应中的作用有待研究。
