Townsend Y, Cranston W I, Hellon R F
Brain Res Bull. 1984 Aug;13(2):335-8. doi: 10.1016/0361-9230(84)90135-7.
In rabbits the third cerebral ventricle was perfused using a push-pull cannula. Prostaglandin E2 concentration in the perfusate was measured by radioimmunoassay. Prostaglandin concentration rose during fever induced by an intraventricular injection of endogenous pyrogen. Both fever and the increased prostaglandin concentration were suppressed by the intraventricular injection of 100 micrograms of the protein synthesis inhibitor, anisomycin. A possible interpretation of the findings is that anisomycin inhibits the formation of phospholipase A2. If this is true, the implication is that phospholipase A2 has a rapid turnover in brain.
在兔子身上，使用推挽式套管对第三脑室进行灌注。通过放射免疫测定法测量灌注液中前列腺素E2的浓度。脑室内注射内源性致热原引起发热时，前列腺素浓度升高。脑室内注射100微克蛋白质合成抑制剂茴香霉素可抑制发热和前列腺素浓度升高。这些发现的一种可能解释是，茴香霉素抑制磷脂酶A2的形成。如果这是真的，那就意味着磷脂酶A2在大脑中具有快速更新率。
