Usui M, Sasaki T, Yagi T, Kobayashi M, Matsuno T, Ishii S, Goto M, Yamawaki S, Ubayama Y, Isu K
Nihon Seikeigeka Gakkai Zasshi. 1984 Aug;58(8):793-801.
This study was undertaken to clarify whether the osteoid forming activity seen in osteosarcoma can be used as an index to show the degree of differentiation of the tumor. Twenty six human osteosarcomas which had been amputated in our clinics were used in this study. The whole slice of the primary tumor was stained by HE and Masson stain. The following observations were microscopically made in the peripheral, transitional and central part of the tumor; 1) the content and maturity of osteoid, 2) the degree of polymorphism and mitotic activity of the tumor cells. In 13 out of 26 cases, the amount and maturity of osteoid gradually increased toward the center of the tumor. On the other hand, the degree of polymorphism and mitotic activity of tumor cells in these cases gradually decreased toward the center. Mitotic activity in the center of the tumor decreased to about half of the peripheral part. The clinical courses of these 13 cases were milder than those in which the gradual increase of the amount and maturity of osteoid toward the center of the tumor were not observed. These results showed that zone formation of osteoid maturity in osteosarcoma can be used as an index to show the degree of differentiation of the tumor.
本研究旨在阐明骨肉瘤中所见的类骨质形成活性是否可作为显示肿瘤分化程度的指标。本研究使用了在我们诊所截肢的26例人类骨肉瘤。原发性肿瘤的全切片用苏木精-伊红（HE）染色和马森三色染色。在肿瘤的外周、过渡和中心部分进行了以下显微镜观察：1）类骨质的含量和成熟度；2）肿瘤细胞的多形性程度和有丝分裂活性。26例中有13例，类骨质的数量和成熟度向肿瘤中心逐渐增加。另一方面，这些病例中肿瘤细胞的多形性程度和有丝分裂活性向中心逐渐降低。肿瘤中心的有丝分裂活性降至外周部分的约一半。这13例的临床病程比未观察到类骨质数量和成熟度向肿瘤中心逐渐增加的病例更为缓和。这些结果表明，骨肉瘤中类骨质成熟度的区域形成可作为显示肿瘤分化程度的指标。
