Sved S, McGilveray I J, Beaudoin N
J Chromatogr. 1978 May 1;145(3):437-44. doi: 10.1016/s0378-4347(00)81373-0.
A rapid, sensitive, accurate method for determination of quinidine in plasma has been developed using ion-pair extraction and high-performance liquid chromatography. The method, which is capable of distinguishing between quinidine and dihydroquinidine, involves acidification of plasma with perchloric acid, extraction with methyl isobutyl ketone and chromatography of the carbonate-washed extract on a silica gel column with a mobile phase of methylene chloride-hexane-methanol--perchloric acid (60:35:5.5:0.1) followed by fluorometric detection. The procedure is sensitive to below 50 ng/ml (coefficient of variation 6.6%) and compares favourably with a standard spectrofluorometric method when tested with plasma from volunteer subjects.
已开发出一种使用离子对萃取和高效液相色谱法测定血浆中奎尼丁的快速、灵敏、准确的方法。该方法能够区分奎尼丁和二氢奎尼丁，包括用高氯酸酸化血浆，用甲基异丁酮萃取，以及将经碳酸洗涤的萃取物在硅胶柱上进行色谱分析，流动相为二氯甲烷 - 己烷 - 甲醇 - 高氯酸（60:35:5.5:0.1），随后进行荧光检测。该方法对低于50 ng/ml的浓度敏感（变异系数为6.6%），在用志愿者血浆进行测试时，与标准荧光分光光度法相比具有优势。
