Russell A O, Patton C M, Kaufmann A F
J Clin Microbiol. 1978 May;7(5):454-8. doi: 10.1128/jcm.7.5.454-458.1978.
The relative efficacy of the card test in the diagnosis of human brucellosis was evaluated by comparison with four other tests: the standard tube agglutination test, centrifugation agglutination test, 2-mercaptoethanol degradation technique, and 2-mercaptoethanol centrifugation agglutination test. A total of 1,701 serum specimens from persons with various degrees of potential exposure to brucella organisms or cross-reactive antigens were used in this study. In comparison with standard tube agglutination results, the card test had a sensitivity of 95.3% and a specificity of 84.1%. The card-test antigen was nonreactive with serum specimens from tularemia patients and cholera vaccinees. Our data indicated that this test measures both immunoglobulin G and immunoglobulin M agglutinins. The value of the card test in the presumptive serological diagnosis of clinical brucellosis in humans appears to be low; however, the card test may be of value in serological surveys to delineate high-risk populations.
通过与其他四项检测方法（标准试管凝集试验、离心凝集试验、2-巯基乙醇降解技术和2-巯基乙醇离心凝集试验）进行比较，评估了卡片试验在诊断人类布鲁氏菌病方面的相对效力。本研究共使用了1701份来自不同程度潜在暴露于布鲁氏菌或交叉反应抗原的人群的血清标本。与标准试管凝集试验结果相比，卡片试验的灵敏度为95.3%，特异性为84.1%。卡片试验抗原与兔热病患者和霍乱疫苗接种者的血清标本无反应。我们的数据表明，该试验可检测免疫球蛋白G和免疫球蛋白M凝集素。卡片试验在人类临床布鲁氏菌病的血清学初步诊断中的价值似乎较低；然而，卡片试验在血清学调查以确定高危人群方面可能具有价值。
