Altwegg M, Bohl E
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Aug;260(1):65-70. doi: 10.1016/s0176-6724(85)80099-7.
In many laboratories the serological diagnosis of brucellosis is based on a tube agglutination test requiring incubation for two days and therefore causing delay in reporting of results. A slide agglutination test using an acidified, rose Bengal colored antigen that can be read after only 4 min was evaluated for its usefulness as a screening test for the presence of agglutinins in human sera. In sera of patients with culturally proven brucellosis or serological evidence for active infection the sensitivity of the slide agglutination test was 100%. Of 97 tube agglutination-negative sera none was positive in the slide test. From these results it was concluded that sera can be tested by the slide agglutination test which is quicker, easier to perform, and less expensive than the tube agglutination test. If positive, it is followed by a tube agglutination test which gives quantitative information and is necessary to follow the course of an infection.
在许多实验室中，布鲁氏菌病的血清学诊断基于试管凝集试验，该试验需要孵育两天，因此会导致结果报告延迟。一种使用酸化的、玫瑰红染色抗原的玻片凝集试验，仅需4分钟即可读取结果，对其作为检测人血清中凝集素存在的筛查试验的实用性进行了评估。在经培养证实患有布鲁氏菌病或有活动性感染血清学证据的患者血清中，玻片凝集试验的敏感性为100%。97份试管凝集试验阴性的血清在玻片试验中均为阴性。从这些结果可以得出结论，血清可以通过玻片凝集试验进行检测，该试验比试管凝集试验更快、更易于操作且成本更低。如果结果为阳性，则随后进行试管凝集试验，该试验可提供定量信息，对于跟踪感染过程是必要的。
