Lyaruu D M, Wöltgens J H, Bervoets T J
J Biol Buccale. 1984 Dec;12(4):287-96.
In culture, 10(-3)M 1-p-bromotetramisole (1-pBTM) inhibited mineralization of in vitro secreted enamel matrix in two-day old neonatal hamster first maxillary molar tooth germs. In contrast, in control tooth germs cultured without the inhibitor, new enamel and dentin matrices were secreted and subsequently mineralized in vitro. It is suggested that the arrested enamel matrix mineralization in vitro is a result of reduced phosphate uptake due to alkaline phosphatase inhibition by 1-pBTM. In addition, 1-pBTM induced accumulation of secretory granules in odontoblasts and ameloblasts, but not in the other cells. This is probably a specific effect of the inhibitor on these cells due to alkaline phosphatase inhibition.
在培养过程中，10⁻³M的1 - 对溴四咪唑（1 - pBTM）抑制了2日龄新生仓鼠第一上颌磨牙牙胚体外分泌的釉质基质的矿化。相比之下，在未添加抑制剂培养的对照牙胚中，新的釉质和牙本质基质被分泌出来，并随后在体外矿化。提示体外釉质基质矿化停滞是由于1 - pBTM抑制碱性磷酸酶导致磷酸盐摄取减少所致。此外，1 - pBTM诱导成牙本质细胞和成釉细胞中分泌颗粒的积累，但在其他细胞中未出现这种情况。这可能是由于碱性磷酸酶受到抑制，该抑制剂对这些细胞产生的特异性作用。
