Ruch J V, Karcher-Djuricic V, Osman M, Meyer J M, Lesot H
J Biol Buccale. 1978 Dec;6(4):267-79.
Sixteen or eighteen day old mouse embryonic first lower molars were treated with 5-bromodeoxyuridine (BrdU) in vitro. --In 16-day old tooth germs cytodifferentiation of both odontoblasts and ameloblasts was inhibited. This inhibition was reversible. --In 18-day old tooth germs, the odontoblasts localized at the top of the principal cusps (post-mitotic cells) differentiated normally and secreted predentin. In these conditions ameloblasts localized in front of functional odontoblasts differentiated, although they were able to incorporate BrdU. --In others experiments, control and BrdU-treated tooth germs were dissociated into enamel organs and pulps. Cultivated associations between either control or BrdU treated pulps with either control or BrdU treated enamel organs were analyzed. It appeared that the primary effect of BrdU might result in its incorporation in the preameloblasts which were no longer able to interact normally with the preodontoblasts.
将16或18日龄小鼠胚胎的第一枚下颌磨牙进行体外5-溴脱氧尿苷（BrdU）处理。——在16日龄的牙胚中，成牙本质细胞和成釉细胞的细胞分化均受到抑制。这种抑制是可逆的。——在18日龄的牙胚中，位于主要牙尖顶部的成牙本质细胞（有丝分裂后细胞）正常分化并分泌前期牙本质。在这些情况下，位于功能性成牙本质细胞前方的成釉细胞分化，尽管它们能够掺入BrdU。——在其他实验中，将对照和经BrdU处理的牙胚解离为釉器和牙髓。分析了对照或经BrdU处理的牙髓与对照或经BrdU处理的釉器之间培养的联合情况。结果表明，BrdU的主要作用可能是使其掺入不再能够与前成牙本质细胞正常相互作用的前成釉细胞中。
