Bronckers A L
J Biol Buccale. 1983 Sep;11(3):195-207.
The effect of 3 oxygen tensions (20%, 50% and 95%) on the pre-eruptive tooth development in vitro was studied biochemically and histologically in 2nd maxillary molars of the hamster. The specific uptakes (uptake per microgram dry weight) of 3H-proline and 45Ca were used as parameters for matrix production and mineralization, respectively. The best results were obtained at 50% O2. Histologically, considerable amounts of predentine, dentine and enamel matrix were found in these molars. Biochemically, appreciable synthesis of matrix and mineralization were measured. In an atmosphere containing 20% O2 less predentine, dentine and enamel matrix were formed. Moreover, production of the matrices occurred in a less regular way. In particular, dentinogenesis in the deeper parts of these molars was affected. The odontoblasts in these areas showed an abnormal and decreased matrix production and eventually dedifferentiated. Biochemically, a lower matrix synthesis and mineralization were measured in these molars.
研究了3种氧张力（20%、50%和95%）对仓鼠上颌第二磨牙体外牙萌出前发育的影响，采用生化和组织学方法进行研究。分别以3H-脯氨酸和45Ca的特异性摄取量（每微克干重摄取量）作为基质产生和矿化的参数。在50%氧气条件下获得了最佳结果。组织学上，在这些磨牙中发现了大量的前期牙本质、牙本质和釉质基质。生化分析表明，基质合成和矿化明显。在含20%氧气的环境中，前期牙本质、牙本质和釉质基质形成较少。此外，基质的产生方式不太规则。特别是，这些磨牙深部的牙本质形成受到影响。这些区域的成牙本质细胞显示出异常且减少的基质产生，最终发生去分化。生化分析表明，这些磨牙中的基质合成和矿化较低。
