Boedts D, De Groote G, Van Vuchelen J
Acta Otolaryngol Suppl. 1984;414:108-14. doi: 10.3109/00016488409122890.
To investigate the possible role of type 1 allergy in OME, the immunoglobulin E (IgE) content was determined in 58 middle ear effusions and matched sera, obtained from children with otitis media with effusion (OME). The IgE levels were assayed for IgE by the Phadebas paper radio immunosorbent test (PRIST). Eight cases (14%) showed elevated serum IgE levels, such that an atopic disorder was very likely. However, in nearly all cases the effusion/serum ratio (E/S) was less than one, showing a higher IgE level in the serum than in the effusion. A modified PAP technique for determining surface IgE on mast cells is presented. Positive results were obtained on nasal mucosa specimens in atopic patients with OME but further research on middle ear mucosa in OME is needed. Our results fail to support the concept of type 1 allergy as a major causative factor in OME.
为研究1型过敏在中耳积液（OME）中的可能作用，测定了58例中耳积液及相应血清中的免疫球蛋白E（IgE）含量，这些样本取自患有渗出性中耳炎（OME）的儿童。采用法德巴斯纸放射免疫吸附试验（PRIST）检测IgE水平。8例（14%）血清IgE水平升高，提示很可能存在特应性疾病。然而，几乎在所有病例中，积液/血清比值（E/S）均小于1，表明血清中的IgE水平高于积液中的水平。本文介绍了一种用于测定肥大细胞表面IgE的改良PAP技术。在患有OME的特应性患者的鼻黏膜标本上获得了阳性结果，但仍需对OME患者的中耳黏膜进行进一步研究。我们的结果不支持1型过敏是OME主要致病因素这一概念。
