Skarin A, Larsson L, Holst E, Mårdh P A
Scand J Urol Nephrol Suppl. 1984;86:71-4.
The fatty acid compositions of forty-five strains of anaerobic curved rods isolated from vaginal content were analysed by gas chromatography. Twenty-six of the strains were of a short Gram-variable type and 19 of a long Gram-negative type. The bacteria were subjected to alkaline saponification, after which the liberated fatty acids were methylated using boron trichloride-methanol. The samples were introduced onto a cross-linked (SE-54) fused silica capillary column. The major peaks in the chromatograms from all strains corresponded to C14:0, C16:0, C18:2, C18:1 and C18:0 fatty acids. Five additional components were seen, three of them identified by mass spectrometry as fatty aldehydes (saturated and unsaturated) of 16 to 18 carbon atoms, whereas the remaining two were not identified. The fatty aldehydes indicated the presence of plasmalogens in the bacterial strains studied. With two exceptions, all of the short rods were distinguishable from the long ones by the presence of one aldehyde and two unidentified compounds, all three eluting between C16:0 and C18:2.
采用气相色谱法分析了从阴道分泌物中分离出的45株厌氧弯曲杆菌的脂肪酸组成。其中26株为短革兰氏可变型，19株为长革兰氏阴性型。对细菌进行碱性皂化处理，然后用三氯化硼 - 甲醇将释放出的脂肪酸甲基化。将样品注入交联（SE - 54）熔融石英毛细管柱。所有菌株色谱图中的主要峰对应于C14:0、C16:0、C18:2、C18:1和C18:0脂肪酸。还观察到另外五个成分，其中三个通过质谱鉴定为16至18个碳原子的脂肪醛（饱和和不饱和），而其余两个未鉴定。脂肪醛表明所研究的细菌菌株中存在缩醛磷脂。除两株外，所有短杆菌都可通过存在一种醛和两种未鉴定化合物与长杆菌区分开来，这三种化合物均在C16:0和C18:2之间洗脱。
