Zhou F, Xu Z, Chen L, Zhu H, Chen X, Wang M
Institute of Microbiology and Epidemiology, Fangtai, Beijing.
Wei Sheng Wu Xue Bao. 1991 Apr;31(2):145-50.
Fatty acid compositions of 7 strains of Rickettsia were analyzed by a on-line GC/MS system. These strains were R. prowazekii E, R. conorii Simko, R. rickettsii R, R. sibirica Barbash and 246, R. sinkiangensis Jinghe, and R. heilungkiangensis 54. The samples were purified by means of the concentrated salt-ether method. There were about 50 peaks in the fatty acid profiles, and 16 of these peaks were determined, i.e. C22:0, C19:0, C18:0, C18:1, C18:2, C17:0, C16:0, C16:1, 3-OH-C14:0, C15:0, C14:0, C13:0, 2-OH-C12:0, C12:0, 2-OH-C10:0, and C11:0. The major fatty acids were the saturated straight chain acids (e.g. C16:0, C18:0, C14:0) and the unsaturated straight chain acids (e.g. C18:1, C18:2, C16:1). Similarities of fatty acid profiles of tested strains were discriminated by the improved Kulik-Vincent method. The result showed that the KV's coefficient of strains Jinghe and 246 was 97.0%, and the KV's coefficient of strains 54 and the others was 81.6-94.6%.
采用在线气相色谱/质谱联用系统分析了7株立克次氏体的脂肪酸组成。这些菌株分别是普氏立克次氏体E株、康氏立克次氏体Simko株、立氏立克次氏体R株、西伯利亚立克次氏体Barbash株和246株、新疆立克次氏体精河株以及黑龙江立克次氏体54株。样品采用浓盐 - 乙醚法进行纯化。脂肪酸图谱中约有50个峰，其中16个峰被鉴定出来，即C22:0、C19:0、C18:0、C18:1、C18:2、C17:0、C16:0、C16:1、3 - OH - C14:0、C15:0、C14:0、C13:0、2 - OH - C12:0、C12:0、2 - OH - C10:0和C11:0。主要脂肪酸为饱和直链酸（如C16:0、C18:0、C14:0）和不饱和直链酸（如C18:1、C18:2、C16:1）。采用改进的库利克 - 文森特法区分受试菌株脂肪酸图谱的相似性。结果表明，精河株和246株的KV系数为97.0%，54株与其他菌株的KV系数为81.6 - 94.6%。
