Zhou F, Chen L, Chen J, Zhu H
Institute of Microbiology and Epidemiology, Beijing.
Wei Sheng Wu Xue Bao. 1990 Dec;30(6):408-16.
Cellular fatty acid compositions of 15 Enterobacteria were analyzed by gas chromatography-mass spectrometry(GC-MS). About 30 fatty acids were detected in chromatograms, and 13 of them were chemically identified, e.i. C11:0, C12:0, C13:0, C14:0, C15:0, 2OH-C14:0, 3OH-C14:0, C16:1, C16:0, aC17:0, delta C17:0, C18:1 and C18:0. The major cellular fatty acids in all fifteen species were C16:0, C18:1, C15:0, C14:0, C16:1, C13:0, 3OH-C14:0 and C18:0. The cellular fatty acids of Enterobacteria species were characterized by normal straight-chain saturated acids and monounsaturated acids, of which the most abundant fatty acid was C16:0. 3OH-C14:0 was found in all strains of Enterobacteria, whereas 2OH-C14:0 was only found in strains of Serratia species. Other unknown compositions also would have been certain characteristics for bacteria. The present paper would provide some of useful reference data for chemotaxonomy and molecular microbiology of Enterobacteria.
采用气相色谱-质谱联用仪（GC-MS）分析了15株肠杆菌的细胞脂肪酸组成。在色谱图中检测到约30种脂肪酸，其中13种经化学鉴定，即C11:0、C12:0、C13:0、C14:0、C15:0、2OH-C14:0、3OH-C14:0、C16:1、C16:0、aC17:0、delta C17:0、C18:1和C18:0。所有15个菌种中的主要细胞脂肪酸为C16:0、C18:1、C15:0、C14:0、C16:1、C13:0、3OH-C14:0和C18:0。肠杆菌菌种的细胞脂肪酸以正常直链饱和酸和单不饱和酸为特征，其中最丰富的脂肪酸是C16:0。在所有肠杆菌菌株中均发现了3OH-C14:0，而2OH-C14:0仅在沙雷氏菌属菌株中发现。其他未知成分也可能是细菌的某些特征。本文将为肠杆菌的化学分类学和分子微生物学提供一些有用的参考数据。
