McGeachie J K, Prendergast F J, Morris P J
Ann R Coll Surg Engl. 1983 Mar;65(2):85-9.
Vein grafts were studied in rats using the incompatible DA and Lewis inbred strains. Allografts were performed in the Lewis-to-DA and DA-to-Lewis combinations with Lewis-to-Lewis isografts serving as controls. A 5 mm segment of iliolumbar vein was placed into a defect in the iliac artery, using micro-surgical techniques. Histological studies of the grafts at 1 hour, 1, 2, 4, 10 and 18 weeks after insertion showed that there was no evidence of rejection in any of the allografts and the tissue changes which occurred were indistinguishable from those of isografts. At the time of grafting, the vein wall consisted of a thin layer of collagen (50 microns thick) with a fine elastic lamina and no endothelium. By 1 week, most of this tissue was necrotic. By 2 weeks an endothelial layer was present and the wall was made of connective tissue, which increased in density by 4 weeks. At 10 weeks, a thick new intima had formed which doubled in thickness by 18 weeks. Electron micrographs revealed that this 'regenerated' intima was composed of smooth muscle cells surrounded by dense collagen and elastin. Sclerotic plaques were evident in some 18 week grafts.
在大鼠中使用不相容的DA和Lewis近交系研究静脉移植物。以Lewis-to-Lewis同基因移植作为对照，进行Lewis-to-DA和DA-to-Lewis组合的同种异体移植。采用显微外科技术将一段5毫米的髂腰静脉置于髂动脉缺损处。对移植后1小时、1周、2周、4周、10周和18周的移植物进行组织学研究，结果显示任何同种异体移植物均无排斥迹象，且所发生的组织变化与同基因移植物的变化无法区分。在移植时，静脉壁由一层薄的胶原层（50微米厚）组成，有一层精细的弹性膜且无内皮。到1周时，大部分这种组织坏死。到2周时出现内皮细胞层，静脉壁由结缔组织构成，到4周时结缔组织密度增加。在10周时，形成了一层厚厚的新内膜，到18周时厚度增加一倍。电子显微镜照片显示，这种“再生”内膜由被致密胶原和弹性蛋白包围的平滑肌细胞组成。在一些18周的移植物中可见硬化斑块。
