Popovic M, Lange-Wantzin G, Sarin P S, Mann D, Gallo R C
Proc Natl Acad Sci U S A. 1983 Sep;80(17):5402-6. doi: 10.1073/pnas.80.17.5402.
Several isolates of human T-cell leukemia/lymphoma virus (HTLV) were transmitted to normal human T cells obtained from the umbilical cord blood of newborns. T cells from seven specimens were immortalized by infection with different HTLV isolates and their properties were compared with those of activated uninfected normal T cells grown in the presence of T-cell growth factor (TCGF) and with those of HTLV-positive neoplastic T-cell lines derived from patients with T-cell malignancies. The HTLV-infected cells generally belonged to a class of mature T cells (OKT4+ and Leu 3A+) and differed from the normal uninfected cells in that they could be propagated in culture indefinitely; possessed altered morphology, including convoluted nuclei and some bi- and multinucleated giant cells; formed large clumps in culture; demonstrated a diminished requirement for TCGF; had an increased density of TCGF receptors; often became completely independent of exogenous TCGF; and expressed HLA-DR determinants. These properties of the HTLV-infected cord blood T cells contrasted to those of uncultured cord blood T cells and of cord blood cells stimulated with mitogen and grown with TCGF but resembled the characteristics of T-cell lines established previously from patients with HTLV-associated T-cell malignancies. This in vitro system offers a unique opportunity to study the basic mechanism involved in abnormal growth and neoplastic transformation of a specific class of human T cells.
几株人类T细胞白血病/淋巴瘤病毒（HTLV）被接种到从新生儿脐带血获取的正常人T细胞中。来自7份标本的T细胞通过感染不同的HTLV分离株而永生化，并将它们的特性与在T细胞生长因子（TCGF）存在下培养的未感染的活化正常T细胞以及来自T细胞恶性肿瘤患者的HTLV阳性肿瘤性T细胞系的特性进行比较。HTLV感染的细胞通常属于一类成熟T细胞（OKT4 +和Leu 3A +），与未感染的正常细胞不同，它们能够在培养中无限增殖；具有形态改变，包括核卷曲以及一些双核和多核巨细胞；在培养中形成大的细胞团；对TCGF的需求减少；TCGF受体密度增加；常常完全不依赖外源性TCGF；并表达HLA - DR决定簇。HTLV感染的脐带血T细胞的这些特性与未培养的脐带血T细胞以及用丝裂原刺激并用TCGF培养的脐带血细胞的特性形成对比，但类似于先前从HTLV相关T细胞恶性肿瘤患者建立的T细胞系的特征。这个体外系统为研究特定类别的人类T细胞异常生长和肿瘤转化所涉及的基本机制提供了一个独特的机会。
