Calvin H I
J Exp Zool. 1978 Jun;204(3):445-52. doi: 10.1002/jez.1402040315.
Selenium is necessary for normal sperm tail development in the rat. The biochemical locus of this requirement was investigated by intratesticular administration of (75Se)SeO3-2. Sperm were labeled primarily in tail keratin, a disulfide-stabilized fraction obtained by extracting isolated tails with sodium dodecyl sulfate (SDS). By incubation in 1% SDS-0.2 mM dithiothreitol, followed by sonication and centrifugation, the 75Se was separated from a residue rich in S and Zn, composed of dense fibers and connecting pieces. The extracted 75Se-labeled material was non-dialyzable, sensitive to pronase, retained most of its bound isotope following carboxymethylation, and displayed only one radioactive component, of 17,000 daltons, during SDS-polyacrylamide gel electrophoresis. It is suggested that the labeled moiety is a selenopolypeptide whose function is critical for normal assembly of the sperm tail.
硒对于大鼠正常精子尾部发育是必需的。通过睾丸内注射（75Se）硒酸根离子（SeO3-2）来研究这一需求的生化位点。精子主要在尾部角蛋白中被标记，尾部角蛋白是一种通过用十二烷基硫酸钠（SDS）提取分离的尾部而获得的二硫键稳定组分。通过在1% SDS - 0.2 mM二硫苏糖醇中孵育，随后进行超声处理和离心，75Se与富含S和Zn的残留物分离，该残留物由致密纤维和连接片组成。提取的75Se标记物质不可透析，对链霉蛋白酶敏感，羧甲基化后保留了大部分结合的同位素，并且在SDS - 聚丙烯酰胺凝胶电泳期间仅显示一个17,000道尔顿的放射性组分。有人提出标记部分是一种硒多肽，其功能对于精子尾部的正常组装至关重要。
