Long S S, Teter M J, Gilligan P H
J Infect Dis. 1983 May;147(5):800-6. doi: 10.1093/infdis/147.5.800.
Biotype and serotype were determined for 473 isolates of Haemophilus influenzae from 399 children. Patients with invasive disease usually had serotypable (86%) and biotype 1 (80%) isolates. Respiratory isolates from well children and children whose illness was not caused by H. influenzae were rarely serotypable (1%) or biotype 1 (8%). Respiratory isolates from children whose illness was possibly or probably related to H. influenzae and isolates from children with cystic fibrosis were more frequently biotype 1, serotypable, or both (P less than 0.01 for all comparisons). Ten (67%) of 15 children with acute otitis media due to H. influenzae had tympanocentesis isolates which were biotype 1; only one isolate was serotypable. Isolates from inflamed conjunctivae had biotypes and serotypes similar to respiratory isolates from well children. Ampicillin resistance was less frequent among biotype 1 isolates (9%) compared with other biotypes (19%) (P less than 0.01). The data suggest that H. influenzae is biochemically heterogeneous and that noncapsular factors, frequently predictable by biotype, contribute to virulence.
对来自399名儿童的473株流感嗜血杆菌分离株进行了生物型和血清型测定。侵袭性疾病患者的分离株通常具有可分型血清型（86%）和生物型1（80%）。健康儿童以及疾病并非由流感嗜血杆菌引起的儿童的呼吸道分离株很少能分型血清型（1%）或为生物型1（8%）。疾病可能或很可能与流感嗜血杆菌相关的儿童的呼吸道分离株以及囊性纤维化患儿的分离株中，生物型1、可分型血清型或两者兼具的情况更为常见（所有比较中P均小于0.01）。15例由流感嗜血杆菌引起急性中耳炎的儿童中，有10例（67%）鼓膜穿刺分离株为生物型1；只有1株可分型血清型。来自炎症性结膜的分离株的生物型和血清型与健康儿童的呼吸道分离株相似。与其他生物型（19%）相比，生物型1分离株中氨苄西林耐药性的发生率较低（9%）（P小于0.01）。数据表明，流感嗜血杆菌在生化方面具有异质性，并且非荚膜因素（通常可由生物型预测）有助于其毒力。
