Ohmichi Y, Nomoto K, Yamada H, Takeya K
Immunology. 1976 Jul;31(1):101-10.
Relationships among tuberculin type hypersensitivity, Jones-Mote type hypersensitivity and activation of helper T cells were studied in AKR mice by means of footpad reaction, migration inhibition test and antibody production against the trinitrophenyl group. (1) Immunization with SRBC in saline, Freund's incomplete adjuvant (FIA) or complete adjuvant (FCA) and fixed-SRBC (FRBC) in FIA- or FCA-induced delayed hypersensitivity as demonstrated by footpad swelling. (2) Migration inhibition was positive in the groups immunized with SRBC or FRBC in FCA, but negative in those immunized with SRBC in saline or FIA or FRBC in FIA. This may suggest that the former has to be assigned to tuberculin type and the latter to Jones-Mote type. (3) Both pre-treatment with BCG and with cyclophosphamide (CY) augmented delayed footpad reaction in the mice immunized with SRBC in saline. However, migration inhibition was positive only in the group pre-treated with BCG. BCG may convert the reaction from Jones-Mote type to tuberculin type, while CY may augment the reaction of Jones-Mote type. (4) FRBC in saline scarcely induced delayed footpad reaction, whereas they activated helper function efficiently. Thus, three types of immunological phenomena attributable to the functions of T cells may depend upon distinct subpopulations of differentiated T cells which are raised by different methods of immunization.
通过足垫反应、迁移抑制试验以及针对三硝基苯基的抗体产生情况，研究了AKR小鼠中结核菌素型超敏反应、琼斯-莫特型超敏反应与辅助性T细胞激活之间的关系。（1）用生理盐水、弗氏不完全佐剂（FIA）或完全佐剂（FCA）中的绵羊红细胞（SRBC）以及FIA或FCA中的固定绵羊红细胞（FRBC）进行免疫，均可诱导足垫肿胀所证明的迟发型超敏反应。（2）在FCA中用SRBC或FRBC免疫的组中迁移抑制呈阳性，但在生理盐水或FIA中的SRBC免疫组或FIA中的FRBC免疫组中迁移抑制呈阴性。这可能表明前者应归为结核菌素型，后者归为琼斯-莫特型。（3）卡介苗（BCG）预处理和环磷酰胺（CY）预处理均增强了生理盐水免疫SRBC小鼠的迟发型足垫反应。然而，只有BCG预处理组的迁移抑制呈阳性。BCG可能将反应从琼斯-莫特型转变为结核菌素型，而CY可能增强琼斯-莫特型反应。（4）生理盐水中的FRBC几乎不诱导迟发型足垫反应，但其能有效激活辅助功能。因此，三种归因于T细胞功能的免疫现象可能取决于通过不同免疫方法产生的分化T细胞的不同亚群。
