经去污剂处理的嗜热四膜虫和衣藻的游动细胞。
Motile detergent-extracted cells of Tetrahymena and Chlamydomonas.
作者信息
Goodenough U W
出版信息
J Cell Biol. 1983 Jun;96(6):1610-21. doi: 10.1083/jcb.96.6.1610.
Abstract
Tetrahymena and Chlamydomonas cells treated with high (0.25-0.5%) concentrations of the detergent Nonidet P-40 in appropriate buffers retain the shape of the intact cells but are devoid of any ciliary activity unless supplied with MgATP. ATP causes them to swim actively, with beat parameters and swimming patterns indistinguishable from those of intact cells. Both types of detergent-extracted cells are completely devoid of ciliary membranes. The Tetrahymena preparations also lack all cellular membranes, whereas cellular membranes remain intact in the Chlamydomonas preparations. Experiments demonstrating the effects of ATP, ADP, vanadate, erythro-9-[3-2-(hydroxynonyl)]-adenine, and Ca++ are described to illustrate the use of these detergent-extracted cells in research on ciliary motility.
摘要
在适当缓冲液中用高浓度（0.25 - 0.5%）去污剂Nonidet P - 40处理的四膜虫和衣藻细胞，保持完整细胞的形态，但除非提供MgATP，否则没有任何纤毛活性。ATP使它们能够活跃游动，其搏动参数和游动模式与完整细胞无异。两种经去污剂处理的细胞都完全没有纤毛膜。四膜虫制剂也缺乏所有细胞膜，而衣藻制剂中的细胞膜保持完整。描述了证明ATP、ADP、钒酸盐、erythro - 9 - [3 - 2 -（羟基壬基）] - 腺嘌呤和Ca++作用的实验，以说明这些经去污剂处理的细胞在纤毛运动研究中的应用。
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