Leiter E, Lutz D, Michlmayr G, Gadner H, Huber C
Wien Klin Wochenschr. 1983 Mar 4;95(5):149-53.
This study aimed to evaluate the molecular weight distribution patterns and the quantitative expression of surface glycoproteins (S-GP) of various differentiation stages of human leucocytes. S-GP were first exposed by treatment with neuraminidase; subsequently they were labelled by galactose-oxidase treatment followed by reduction with 3H-sodium borohydride. Labelled S-GP were separated on polyacrylamide gels in the presence of SDS and were visualized by means of fluorography. A total of 8 major S-GP bands with apparent molecular weights of 230 000, 215 000, 200 000, 185 000, 175 000, 150 000, 125 000 and 110 000 daltons were identified. All of these S-GP were already expressed at the level of pluripotent myelopoietic stem cells but different in their relative expression during further cellular maturation.
本研究旨在评估人白细胞不同分化阶段的表面糖蛋白（S-GP）的分子量分布模式及定量表达。首先用神经氨酸酶处理使S-GP暴露；随后用半乳糖氧化酶处理，再用3H-硼氢化钠还原进行标记。标记的S-GP在十二烷基硫酸钠存在下于聚丙烯酰胺凝胶上分离，并通过荧光自显影法显现。共鉴定出8条主要的S-GP条带，其表观分子量分别为230 000、215 000、200 000、185 000、175 000、150 000、125 000和110 000道尔顿。所有这些S-GP在多能骨髓造血干细胞水平就已表达，但在细胞进一步成熟过程中的相对表达有所不同。
