Raji A R, Bowden R E
J Bone Joint Surg Br. 1983 Aug;65(4):478-92. doi: 10.1302/0301-620X.65B4.6603461.
Apart from preliminary notices of present work, previous reports of experimental and clinical trials of the effects of a high-peak pulsed electromagnetic field (PEMF) on degeneration and regeneration of peripheral nerves lacked statistical analysis. Therefore, we designed experiments with standardised operative, histological, cytological and morphometric techniques to assess the effect of PEMF on lesions of the common peroneal nerves in paired male rats matched for age, environmental conditions and level and type of lesion. One of two types of lesion was induced in the left common peroneal nerve: in 12 pairs of rats the nerve was crushed just above the knee and in the remaining 12 pairs the nerve was cut and immediately sutured at the same level. The right common peroneal nerve of each rat served as a control. Animals received 15 minutes of PEMF produced by a Diapulse machine or sham treatment daily for periods ranging from three and a half days to eight weeks after injury. Healthy nerves were unaffected, but after damage there were statistically significant differences between PEMF treated and sham treated rats. PEMF accelerated the recovery of injured limbs and the degeneration, regeneration and maturation of myelinated axons; epineural, perineural and intraneural fibrosis was reduced; and the luminal cross-sectional area of intraneural vessels increased after both types of lesion. Findings are discussed and the need for clinical trials is stressed.
除了关于当前工作的初步报告外，先前有关高峰值脉冲电磁场（PEMF）对外周神经退变和再生影响的实验及临床试验报告均缺乏统计分析。因此，我们采用标准化的手术、组织学、细胞学和形态测量技术设计实验，以评估PEMF对年龄、环境条件以及损伤程度和类型相匹配的雄性配对大鼠腓总神经损伤的影响。在左侧腓总神经上诱导产生两种类型的损伤之一：在12对大鼠中，神经在膝盖上方被挤压；在其余12对大鼠中，神经被切断并立即在同一水平缝合。每只大鼠的右侧腓总神经作为对照。动物在受伤后3.5天至8周的时间段内，每天接受由Diapulse机器产生的15分钟PEMF治疗或假治疗。健康神经未受影响，但损伤后，接受PEMF治疗的大鼠与接受假治疗的大鼠之间存在统计学上的显著差异。PEMF加速了受伤肢体的恢复以及有髓轴突的退变、再生和成熟；减少了神经外膜、神经束膜和神经内膜的纤维化；并且在两种类型的损伤后，神经内血管的管腔横截面积均增加。对研究结果进行了讨论，并强调了进行临床试验的必要性。
